Objective Silver nanoparticles synthesized usingRhodomyrtus tomentosa leaf extract and silk sericin were used to functionalize carbopol 940 gel for topical applications. Results UV-vis spectra presented surface plasmon resonance at 426 nm, transmission electron microscopy revealed that nanoparticles were spherical with an average size of 25-50 nm. X-ray diffraction presented crystalline silver nanoparticles with zeta potential of approximate to - 30 mV. FTIR spectra showed a reduction of silver nitrate indicated by the shift in -OH at 2958 cm(-1). The silver nanoparticle demonstrated broad-spectrum antimicrobial activity against Gram-positive, Gram-negative and fungi with MIC ranging between 0.26 and 2.10 mu g mL(-1), respectively. MIC of hydrogel ranged between 1.05-2.10 mu g mL(-1)with cell viability of 89%. Spreadability and extrudability of gel were 9.3 +/- 0.85 s and 19.85 +/- 0.03%, respectively with first order of fickian diffusion. Conclusions The silver nanoparticle gel exhibited an effective antimicrobial property, hence can be exploited for topical applications.