Mycelial adhesion affects cell growth and the production of water-soluble extracellular yellow pigment (EYP) in submerged fermentation withMonascus ruberCGMCC 10910. Two nitrates, NaNO(3)and KNO3, were used as nitrogen sources for mitigating mycelial adhesion and improving the production of EYP in this study. The results showed that the adhesion of mycelia in the fermentation broth significantly decreased by adding 5 g/L NaNO3, which prevented mycelia from attaching to the inner wall of the Erlenmeyer flask. It was suggested that NaNO(3)reduced the total amount of extracellular polysaccharides, increased extracellular proteins, and decreased the viscosity of the fermentation broth. Scanning electron microscopy (SEM) analysis revealed that the mycelial morphology was shorter and more dispersed and vigorous under NaNO(3)conditions than under the control conditions. The biomass increased by 49.2% and 45.4% with 5 g/L NaNO(3)and 6 g/L KNO(3)treatment, respectively, compared with that of the control, and the maximum production of EYP was 267.1 and 241.8 AU(350), which increased by 70.0% and 53.9% compared with that of the control, respectively. Simultaneously, the ratios of intracellular yellow pigment to orange pigment increased significantly with 5 g/L of NaNO(3)addition (p < 0.05). Genetic analysis found that the expression levels of the key genes forMonascuspigment biosynthesis were significantly upregulated by NaNO(3)addition (p < 0.05 orp < 0.01). This study provides an effective strategy for the production of water-solubleMonascusyellow pigments.