Solid-phase immunoassays are based on the use of appropriate ligands and solid supports. In order to investigate the influence of the solid support on the interaction process, a predictive model of antibody/antigen interaction has been developed. Parameters of this model are estimated from dedicated experiments like surface plasmon resonance and affinity chromatography by using least-squares optimization techniques. Affinity chromatography is of great interest because unlike surface plasmon resonance, it enables the estimation of kinetic parameters on any solid support used in immunoassays. The approach described above is illustrated through the kinetic modeling of the interactions between free prostate-specific antigen (FPSA) and an anti-FPSA antibody.