화학공학소재연구정보센터
Current Microbiology, Vol.77, No.6, 1104-1110, 2020
Ochrobactrum soli sp. nov., Isolated from a Korean Cattle Farm
A Gram stain negative, motile, non-spore-forming, rod-shaped, strictly aerobic, beige-pigmented bacterium, designated strain BO-7(T), was isolated from soil of cattle farm, in Seosan, Republic of Korea. On the basis of 16S rRNA gene sequencing, strain BO-7(T) clustered with species of the genus Ochrobactrum and appeared closely related to O. haematophilum CCUG 38531(T) (98.9%), O. daejeonense KCTC 22458(T) (98.1%), O. rhizosphaerae DSM 19824(T) (98.1%), O. pituitosum DSM 22207(T) (98.0%), and O. pecoris DSM 23868(T) (98.0%). The digital DNA-DNA hybridization and average nucleotide identity between strain BO-7(T) and the closely related strains were 21.9-39.1%, 78.5-89.5%, respectively, indicating that BO-7(T) is a novel species of the genus Ochrobactrum. The DNA G + C content of the genomic DNA was 57.1 mol%, and ubiquinone Q-10 was the predominant respiratory quinone. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethyl-ethanolamine, di-phosphatidylglycerol, the major polyamines were spermidine, putrescine, and sym-homospermidine. The major cellular fatty acids (> 5%) were C-16:0, C-19:0 cycle omega 7c, and C-18:1 omega 7c and/or C-18:1 omega 6c (summed feature 8). ANI calculation, digital DNA-DNA hybridization, physiological and biochemical characteristics indicated that strain BO-7(T) represents a novel species of the genus Ochrobactrum, for which the name Ochrobactrum soli sp. nov. is proposed. The type strain is BO-7(T) (= KACC 19676(T) = LMG 30809(T)).