We report the draft genome sequence ofFructobacillus tropaeoliCRL 2034, a strain isolated from ripe fig in Tucuman province, Argentina. The interest in studying the genome of this fructophilic lactic acid bacterium strain was motivated by its ability to produce high levels of mannitol from fructose. This polyol has multiple industrial applications; however, it is mainly used as low calorie sugar in the food industry. The assembled genome of this strain consists of a 1.66-Mbp circular chromosome with 1465 coding sequences and a G+C content of 44.6%. The analysis of this genome supports the one step reaction of fructose reduction to mannitol by the mannitol 2-dehydrogenase enzyme, which together with a fructose permease, were identified as involved in mannitol synthesis. In addition, a phylogenetic analysis was performed including otherLeuconostocaceaemembers to which theFructobacillusgenus belongs to; according to the 16S rRNA gene sequences, the strain CRL 2034 was located in theFructobacillusclade. The present genome sequence could be useful to further elucidate regulatory processes of mannitol and other bioactive metabolites and to highlight the biotechnological potential of this fruit-originFructobacillusstrain.