Bone reconstruction remains an important challenge today in several clinical situations, notably regarding the control of the competition occurring during proliferation of osteoblasts and fibroblasts. Polystyrene and polylactide are reference materials in the biomedical field. Therefore, it could be expected from the literature that clear correlations have been already established between the behavior of fibroblasts or osteoblasts and the surface characteristics of these materials. After an extensive analysis of the literature, although general trends could be established, our critical review has highlighted the need to develop a more in-depth analysis of the surface properties of these materials. Moreover, the large variation noticed in the experimental conditions used for in vitro animal cell studies impairs comparison between studies. From our comprehensive review on this topic, we have suggested several parameters that would be valuable to standardize to integrate the data from the literature and improve our knowledge on the cell-material interactions.