In the present investigation, isolation and determination of a new strain of Acidithiobacillus ferridurans SBU-SH2 from an Iranian sulfur hot spring are carried out and then it is used to evaluate the performance of uranium bioleaching from a low-grade natural resource host. The efficiency of uranium recovery is studied by considering the influence of experimental factors like pulp density, pH value, temperature, and source of energy. Experimental tests reveal that more than 96% of uranium is recovered at 35 degrees C, initial pH 2.5, agitation speed of 120 rpm, and pulp density of 5% in about 1 week.