Sulfonated poly (aryl ether ketone sulfone) polymers (SPAEKS-PSA X) with the comb-shaped structure were prepared by grafting with 1, 3-propanesultone (PSA). The successful synthesis of SPAEKS-PSA X was confirmed by the FT-IR spectra. The morphology of the membrane was investigated by the small angle X-ray scattering and the transmission electron microscopy. The nanophase separation was brought in a comb-shaped structure between the main chains and the propyl sulfonic acid groups. The SPAEKS-PSA 15 membrane exhibited the highest conductivity of 101 mS cm(-1) under 80 degrees C and 100% relative humidity conditions (100% RH). In addition, the methanol permeability coefficients of SPAEKS-PSA X membranes (7.56 x 10(-7) to 9.44 x 10(-7) cm(2) s(-1)) were much lower compared to Nafion (R) 117 (22.37 x 10(-7) cm(2) s(-1)). Meanwhile, the SPAEKS-PSA X membranes also displayed excellent mechanical properties (tensile modulus >30 MPa), thermal, oxidative stabilities and fuel cell performance. Considering the parameters above, especially the elevated proton conductivities as well as high methanol resistance, the comb-shaped structure is a promising design for DMFCs applications. (C) 2019 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.