The current study aims to explore the opportunities of using the high viscous biofuel namely Wheat Germ oil (WGO) in a twin cylinder CI engine. High viscous fuels suffer from improper atomization leading to poor combustion and higher smoke emission. To address this problem, various techniques namely transesterification, fuel ionization and hydrogen induction were studied. WGO was converted to its ester which reduced the viscosity. Fuel ionization increases the vibrational frequency of the molecules, weakens the bonds and converted to ions, which increases the dispersion rate during injection and improves the combustion subsequently. Hydrogen is having faster flame speed and higher calorific value aids in combustion enhancement at its knock limited levels. The twin cylinder tractor engine selected for this experiment runs at a constant speed of 1500 rpm. The engine was run using diesel to achieve the preferred warm-up condition in order to use WGO, which hada cold starting problem. Tests were conducted with wheat germ biodiesel (WGBD), WGO with permanent magnet (PM), electromagnet (EM) and the combination of PM and EM-based fuel ionization system and finally WGO with hydrogen induction rates of 2%, 4.3%, 6.7% and 10.3% at maximum engine load condition. It is observed that all the techniques improved the performance of WGO. Among the techniques tested, hydrogen induction displayed better results in terms of performance and emission characteristics with a slight penalty in NO emission. (C) 2019 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.