Miniaturization of devices, combined with other features such as portability, proneness to automation, rapid performance, amenability to integration, multiplexing, and cost-effectiveness, is rapidly increasing for various sensing and energy harvesting applications. One such emerging area is the development of microfluidic fuel cell on cellulose papers, which has enormous scope to optimize its performance. This is primarily because such devices eliminate the need for membranes as well as external pumps since they have built-in colaminar flow embedded capillaries. Such peripherals are usually used in conventional microfluidic fuel cells, which are fabricated using methods like photolithography, PDMS lithography, and 3D printing. This paper presents investigations on microfluidic paper-based fuel cells (MPFCs) with different cellulose absorbent pads for their performance optimization. Herein, the MPFC utilizes formic acid as fuel, oxygen from quiescent air as oxidant, and sulfuric acid as electrolyte for conducting ionic exchange under colaminar flow. The electrodes are realized through simple pencil strokes depositing a thin layer of graphite. The porous graphite electrodes act as diffusion agents breathing oxygen directly from the atmospheric air. Such an MPFC configuration, costing less than US $1, was optimized to achieve maximum energy density by examining various combinations of absorbent pads with different grades of cellulose papers. It is seen that the maximum open circuit potential is 0.46 V, while the maximum current and power densities are 1505.66 mu Acm(-2) and 173.97 mu Wcm(-2), respectively, with a grade 6 absorbent pad. Such performance can be further enhanced by investigating MPFCs with various graphite pencils with a diverse number of strokes at different concentration levels.