Mono- and bifunctional hybrid core-shell particles were used for enzyme immobilization. Ideal conditions for enzyme immobilization were evaluated based on monofunctional poly(2-dimethylamino)ethyl methacrylate-modified particles. Then, the obtained results were transferred to a bifunctional Janus particle system, which should simultaneously enable enzyme immobilization, switchable separation, and reuse of the enzyme. Finally, application of the system was exemplarily demonstrated by multiple discoloration of textile industry process water with laccase and a cost estimation is presented.