Colorimetric assay of tyrosinase inhibition using melanocyte laden hydrogel fabricated by digital light processing printing

Hojung Kwak; Sungchul Shin; Jinho Hyun

Journal of Industrial and Engineering Chemistry, Vol.84, 252-259, April, 2020

DOI10.1016/j.jiec.2020.01.006 Export Citation

Colorimetric assay of tyrosinase inhibition using melanocyte laden hydrogel fabricated by digital light processing printing

Hojung Kwak¹, Sungchul Shin¹, and Jinho Hyun^{1, 2, †}

¹Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea
²Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea

E-mail:

Colorimetric assay of melanogenesis inhibition was demonstrated with polyethylene glycol tetraacrylate (PEG-4A) hydrogel structures embedding melanocyte cells. The cell laden PEG-4A hydrogel structure was prepared by digital light processing printing using biocompatible visible range of light. The melanocyte in PEG-4A hydrogel structure showed a high cell compatibility and expressed melanin changing a color of hydrogel to black. The inhibition of melanin expression was evaluated by injecting a different concentration of arbutin and kojic acid to the cell laden hydrogel structure in a 3D printed microfluidic chip. The tyrosinase inhibitors prevented a color change of the hydrogel pad according to the dosage showing a similar detecting efficiency to a commercial melanogenesis assay kit.

Keywords:Colorimetric assay;Melanocyte;Cell-laden hydrogel;Tyrosine inhibitors;Digital light processing;3D printing;Polyethylene glycol tetraacrylate

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[2] Huang L, Liu MY, Huang HY, Wen YQ, Zhang XY, Wei Y, Biomacromolecules, 19(6), 1858 (2018)

[3] D'Orazio J, Jarrett S, Amaro-Ortiz A, Scott T, Int. J. Mol. Sci., 14(6), 12222 (2013)

[4] Sulaimon SS, kitchell BE, Vet. Dermatol., 14, 57 (2003)

[5] Westerhof W, Pigm. Cell. Res., 19, 183 (2006)

[6] Hearing VJ, Jimenez M, Int. J. Biochem., 19, 1141 (1987)

[7] Cabanes J, Chazzara S, Garcia-carmona F, J. Pharm. Pharmacol., 46, 982 (1994)

[8] Ito S, Wakamatsu K, J. Dermatol. Sci., 80, 18 (2015)

[9] Sugimoto K, Nishimura T, Nomura K, Sugimoto K, Kuriki T, Chem. Pharm. Bull., 51, 798 (2003)

[10] Saeedi M, Eslamifar M, Khezri K, Biomed. Pharmacother., 110, 582 (2019)

[11] Shimada Y, Tai H, Tanaka A, Ikezawa-Suzuki I, Takagi K, Yoshida Y, Yoshie H, J. Periodontol., 80, 317 (2009)

[12] Gasowska-Bajger B, Wojtasek H, J. Agric. Food Chem., 64, 5417 (2016)

[13] Ruzza P, Serra PA, Fabbri D, Dettori MA, Rocchitta G, Delogu G, Eur. J. Med. Chem., 126, 1034 (2017)

[14] Ao H, Qian Z, Zhu Y, Zhao M, Tang C, Huang Y, Feng H, Wang A, Biosens. Bioelectron., 86, 542 (2016)

[15] Honisch C, Osto A, de Matos AD, Vincenzi S, Ruzza P, Food Chem., 305, 125506 (2020)

[16] Lin TE, Bondarenko A, Lesch A, Pick H, Cortes-Salazar F, Girault HH, Angew. Chem.-Int. Edit., 55, 3813 (2016)

[17] Wang L, Gan ZF, Guo D, Xia HL, Patrice FT, Hafez ME, Li DW, Anal. Chem., 91, 6507 (2019)

[18] Ciui B, Martin A, Mishra RK, Brunetti B, Nakagawa T, Dawkins TJ, Lyu M, Cristea C, Sandulescu R, Wang J, Adv. Healthc. Mater., 7, 170126 (2018)

[19] Chai L, Zhou J, Feng H, Tang C, Huang Y, Qian Z, ACS Appl. Mater. Interfaces, 7, 23564 (2015)

[20] Yan X, Li H, Zheng W, Su X, Anal. Chem., 87, 8904 (2015)

[21] Sun J, Mei H, Wang S, Gao F, Anal. Chem., 88, 7372 (2016)

[22] Liu B, Huang P, Li J, Wu F, Sens. Actuators B-Chem., 251, 836 (2017)

[23] Deng H, Lin X, He S, Wu G, Wu W, Yang Y, Lin Z, Peng H, Xia X, Chen W, Microchim. Acta, 186, 301 (2019)

[24] Zhang L, Deng H, Lin F, Xu X, Weng S, Liu A, Lin X, Xia X, Chen W, Anal. Chem., 86, 2711 (2014)

[25] Kim H, Choi J, Cho J, Kim S, Lee Y, Bioorg. Med. Chem. Lett., 14, 2843 (2004)

[26] Kahn V, Pigm. Cell. Res., 8, 234 (1995)

[27] Dan-Ning H, Photochem. Photobiol., 84, 645 (2008)

[28] Chung S, Lim G, Lee J, Sci. Rep., 9, 780 (2019)

[29] McKee C, Beeravolu N, Brown C, Cruet M, Chaudhry GR, Appl. Mater. Today, 100474 (2019).

[30] Lee S, Ki C, Carbohydr. Polym., 229, 115555 (2020)

[31] Grenier J, Duval H, Barou F, Lv P, David B, Letourneur D, Acta Biomater., 94, 195 (2019)

[32] Jin R, Teixeira M, Krouwels A, Dijkstra PJ, van Blitterswijk CA, Karperien M, Feijen J, Acta Biomater., 6, 1968 (2010)

[33] Huynh CT, Liu F, Cheng Y, Coughlin KA, Alsberg E, ACS Appl. Mater. Interfaces, 10, 25936 (2018)

[34] Wang J, Kumar H, Tian Z, Jin X, Holzman JF, Menard F, Kim K, ACS Appl. Mater. Interfaces, 10, 26859 (2018)

[35] Lee S, Tong X, Yang F, Acta Biomater., 10, 4167 (2014)

[36] Shin S, Kwak H, Hyun J, ACS Appl. Mater. Interfaces, 10, 23573 (2018)

[37] Wu GH, Hsu SH, J. Med. Biol. Eng., 35, 285 (2015)

[38] Sriram G, Alberti M, Dancik Y, Wu B, Wu R, Feng Z, Ramasamy S, Bigliardi PL, Bigliardi-Qi M, Wang Z, Mater. Today, 21, 326 (2018)

[39] Hao Y, Shih H, Munoz Z, Kemp A, Lin CC, Acta Biomater., 10, 104 (2014)

[40] Kwak HJ, Shin SC, Lee HJ, Hyun JH, J. Ind. Eng. Chem., 72, 232 (2019)

[41] Rubinstein M, Colby RH(Eds.), Polymer physics, Oxford University Press, pp.259 2003.

[42] Wen JH, Vincent LG, Fuhrmann A, Choi YS, Hribar KC, Taylor-Weiner H, Chen SC, Engler AJ, Nat. Mater., 13(10), 979 (2014)