Synthesizing tubes of large diameters is important for materials science and nanotechnology but remains a largely unsolved challenge. We report herein an approach to program the width of DNA helical tubes by controlling the rigidity and curvature of repeating units via single-stranded bricks strategy. The rigidity of repeating units can be controlled by its thickness, and the curvature can be tuned by double-helical twist density. A single-step annealing allows for the fabrication of helical tubes with defined chirality and widths ranging from similar to 50 to similar to 550 nm.