Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA-RNA interface between two structural modules

Tsuruga R; Uehara N; Suzuki Y; Furuta H; Sugiyama H; Endo M; Matsumura S; Ikawa Y

Journal of Bioscience and Bioengineering, Vol.128, No.4, 410-415, 2019

DOI10.1016/j.jbiosc.2019.04.003 Export Citation

Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA-RNA interface between two structural modules

Tsuruga R, Uehara N, Suzuki Y, Furuta H, Sugiyama H, Endo M, Matsumura S, Ikawa Y

Bimolecular ribozymes derived by physical dissection of unimolecular ribozymes consisting of two structural modules are promising platforms for the design and construction of assembled RNA nanostructures. Unit RNAs to be assembled intermolecularly into one-dimensional (1D) oligomers are designed by reconnecting the two structural modules in a manner different from the parent ribozymes. This strategy was applied to the Tetrahymena group I ribozyme. We constructed 1D ribozyme oligomers the assembly of which was observed by atomic force microscopy (AFM) and also controlled rationally to design a heterooctamer by differentiating the interface between the two modules. (C) 2019, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Catalytic RNA;Group I intron;Ribozyme;RNA nanostructure;RNA nanotechnology