Magnesium materials exhibit promising prospects in clinical applications due to their biodegradability. Herein AZ31 magnesium alloy sample is scrutinized as a potential example of a biodegradable ureteral stent material in artificial urine (AU) without and with different doses from amoxicillin (AMX). The study is carried out using open circuit potential (OCP), electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization (PDP) measurements. The results disclose that AMX is indeed a professional inhibitor for A231 alloy degradation in AU fluid with a maximum efficiency of 93.6% at 1.0 mM dose after 8 days immersion. The enhanced protection capability of AMX is due to the high tendency of its molecules to be adsorbed on AZ31 alloy surface which is well described following Langmuir adsorption Isotherm. Results are in good agreement with the calculated highest occupied molecular orbitals (HOMO) and lowest unoccupied molecular orbitals (LUMO) energies of . AMX. A simple nano-Pd modified carbon paste electrode is fabricated and proved to be sensitive and selective in situ electrochemical sensor to monitor AMX during the alloy degradation in AU fluid using cyclic voltammetry technique.