The electronic performance of conjugated polymers depends on the microstructure of the polymer films. A percolated network morphology with high crystallinity, ordered intermolecular packing and long-range order is beneficial for charge transport. In recent reports, some conjugated polymers have been shown to exhibit liquid crystallinity. The appearance of liquid crystalline ordering provides a new solution to solve the difficulties in microstructure manipulation. In this review, we summarize how liquid crystallinity can assist molecular arrangement and guide long-range orientation during film processing, leading to high charge mobility. We expect that this article could draw more attention to the liquid crystallinity of conjugated polymers.