Encouraged by the diversity of n-dodecane chemical mechanisms currently available, this investigation focuses on analysing the impact of using different fuel oxidation schemes on the diesel-like Engine Combustion Network (ECN) Spray A flame structure, simulated by means of an Unsteady Flamelet Progress Variable (UFPV) combustion model. The present research discusses systematically the characteristics of four n-dodecane chemical mechanisms in perfectly stirred reactors and counterflow laminar diffusion flames (flamelets) before the final evaluation in turbulent reacting sprays in order to describe the effects of adding different physical levels of complexity to the ignition of the mixtures. In addition, this analysis is complemented with the description of the effect of the boundary conditions on the flame structure. Results evidence the extreme importance of the low temperature chemistry including the period for which the cool flame extends. The different prediction of this stage between mechanisms leads to noticeable different laminar flame structures which in turn produce substantially distinct turbulent flames, especially in the vicinity of the lift-off length (LOL) in terms of reactivity and positioning in the Z-T map. Finally, simulations confirm the strong effect of the boundary conditions, especially for the ambient temperature, on the ignitable mixtures which directly impacts on the soot precursors formation. (C) 2019 The Combustion Institute. Published by Elsevier Inc. All rights reserved.