This novel study is focused on estimating the electrohydrolysis pretreatment conditions using DC current (voltage and time) for enhancing the biodegradability of rice straw. The electrohydrolysis pretreatment was performed using graphite electrode with intermittent mode of input voltage fluctuating from 10 to 30 V (voltage study) and in continuous mode for 15-80 min (time study). The result implicated that electrohydrolysis pretreatment with 25 V and 60 min time achieved the maximum solublization (55%). The Batch tests (1 L) were performed to explore the accelerated biomethane production from enhanced solublization and to optimize the food microorganisms ratio. The different FPM (0.5-3) ratios showed the cumulative methane production ranging from 236 to 319 mL/g-VSadded (control -224.01 mL/g-VSadded). Compositional characterization (FESEM, XRD and FTIR) studies showed the efficacy in disrupting the lignin layer and reducing the cellulose crystallinity of rice straw. The effect of pretreatment on methane production was also kinetically analysed by modified Gompertz, logistic and transference function models. (C) 2019 Elsevier Ltd. All rights reserved.