In the present research, we developed a novel non-enzymatic fructose sensor by platinum decorated graphene oxide nanocomposite based cyclic voltammetry. This sensor can detect fructose in alkalized medium which converts fructose into oxidizable glucose. The dynamic range of trace detection is within 0.05-0.50 mM and the detection limit was achieved around 10 mu M even in the presence of HPO42- and Cl- as interferent ions. The electrochemical signal from the fructose can be significantly discriminated from that of lactose and sucrose, leaving glucose to be further distinguished in neutral medium. This non-enzymatic fructose sensing approach potentially provides a novel strategy to measure the concentration of fructose in biological fluids with potential application for clinical diagnostics and quality control in food industry.