trans-Aconitic acid (TAA) is an unsaturated tricarboxylic acid often found in natural sources like cane molasses, beet roots, and so forth. Because of potential applications as plasticizers, biodegradable polyesters for tissue engineering, acidulants in food processing, wetting agents in surfactants, and as drugs in neuromedical therapies, its production has got researchers' attention. Fermentation is the promising and green route for the continuous production of TAA that could meet industrial demands. However, separation of biotechnologically produced TAA from fermentation media is a challenging task. In this work, the effect of diluents on liquid-liquid extraction of TAA was investigated using six diluents of varied chemical nature, alcohols (lauryl alcohol and benzyl alcohol), ketones (cyclohexarione and MIBK), and inactive hydrocarbons (benzene & heptanes) with tri-n-butyl phosphate (TBP). The reactive extraction equilibrium was analyzed using parameters such as distribution coefficient (K-D), loading ratio (Z > 1), and extraction efficiency (E).The obtained K-D values lies in the range of 0.001-9.483, whereas maximum extraction achieved was 90.46% in MIBK with TBP 50% (v/v). In addition, it was also observed that physiochemical parameters of diluents like dielectric constant (K), dipole moment (mu), and Dimroth-Reichardt empirical parameter (E-T) have a significant effect on extraction efficiency.