Fuel cells are among the promising approaches for achieving green technology for energy conversion. Glucose is a ubiquitous good candidate for such devices as a high-energy-density fuel. Herein, we report a facile and simple approach to fabricate an efficient Pd-based electrocatalyst for glucose oxidation reaction supported on indium/tin oxide substrates by electrochemical deposition. This approach involves enhancing the electrochemical performance of Pd nanoparticles by using Pd/Ce2O3 nanocomposite instead of single Pd nanoparticles. The synthesized electrocatalysts are characterized for structural and morphological properties using X-ray diffraction analysis, energy dispersive spectroscopy and scanning electron microscopy. Their electrochemical performance is scrutinized using cyclic voltammetry, electrochemical impedance spectroscopy and chronoamperometry. The results indicate that Ce2O3 has a promoting effect for Pd nanoparticles towards glucose oxidation reaction. The efficacy of the electrocatalyst fabricated from Pd/Ce2O3 nanocomposite as a function of glucose concentration indicates that this catalyst is sensitive even for the very low mu M glucose concentration range.