Synthesis of Acetins from Glycerol using Lipase from Wheat Extract

Pradima J; Rajeswari M Kulkarni; Archna Narula; Sravanthi V; Rakshith R; Nawal Rabia Nizar

Korean Chemical Engineering Research, Vol.57, No.4, 501-506, August, 2019

DOI10.9713/kcer.2019.57.4.501 Export Citation

Synthesis of Acetins from Glycerol using Lipase from Wheat Extract

Pradima J^†, Rajeswari M Kulkarni, Archna Narula, Sravanthi V, Rakshith R, and Nawal Rabia Nizar

Department of Chemical Engineering, M S Ramaiah Institute of Technology, MSR Nagar, Bangalore-560054, Karnataka, India

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New technology-driven biocatalysts are revolutionizing the biochemical industries. With maximum utilization of renewable feedstock, biocatalysts have been the basis for a major breakthrough. Lipases are the most widely established catalysts used for hydrolysis, esterification and transesterification reactions. In this research, a biochemical process that combines extraction of lipase enzyme from germinated wheat seeds and its application to valorize glycerol to acetins by esterification is presented. Acetins are among highly rated, value-added products derived from glycerol. The favorable conditions for the enzymatic conversion of glycerol were observed as glycerol to acetic acid molar ratio (1:5), reaction temperature (40 °C) and the amount of enzyme (20% v/v). 65.93% of glycerol conversion was achieved for duration of 15 h with the use of tert-butanol solvent. This method proposes to explore the viability of a biological route to convert glycerol derived from biodiesel industry to acetins with further streamlining.

Keywords:Glycerol;Fuel additives;Enzyme catalysts;Kinetics;Lipase

[References]

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[2] Sharma A, Ghosh A, Pandey RA, Mudliar SN, Korean J. Chem. Eng., 53, 216 (2015)

[3] Shafiei A, Rastegari H, Ghaziaskar HS, Yalpani M, Biofuel Research J., 4(1), 565 (2017)

[4] Bedogni GA, Acevedo MD, Aguzin F, Okulik NB, Padro CL, Environ. Technol., 39(15), 1955 (2018)

[5] Testa ML, La Parola V, Liotta LF, Venezia AM, J. Mol. Catal. A-Chem., 367, 69 (2013)

[6] Costa IC, Itabaiana I, Flores MC, Lourenco AC, Leite SG, de M.e Miranda LS, Leal IC, de Souza RO, J. Flow Chem., 3(2), 41 (2013)

[7] Liao XY, Zhu YL, Wang SG, Chen HM, Li YW, Appl. Catal. B: Environ., 94(1-2), 64 (2010)

[8] Ghaziaskar HS, Afsari S, Rezayat M, Rastegari H, J. Supercrit. Fluids, 119, 52 (2017)

[9] Pradima J, Kulkarni MR, Resource-Efficient Technologies., 3(4), 394 (2017)

[10] Cahyono RB, Mufrodi Z, Hidayat A, Budiman A, ARPN J. Appl. Sci., 11(8) (2016)

[11] Costa BOD, Decolatti HP, Legnoverde MS, Querini CA, Catal. Today, 289, 222 (2017)

[12] Zhou LM, Nguyen TH, Adesina AA, Fuel Process. Technol., 104, 310 (2012)

[13] Sun JY, Tong XL, Yu LH, Wan J, Catal. Today, 264, 115 (2016)

[14] Veluturla S, Archna N, Rao DS, Hezil N, Indraja IS, Spoorthi S, Biofuels, 9(3), 305 (2018)

[15] Testa ML, La Parola V, Mesrar F, Ouanji F, Kacimi M, Ziyad M, Liotta LF, Catalysts, 9(2), 148 (2019)

[16] Asmat S, Husain Q, Azam A, RSC Advances, 7(9), 5019 (2017)

[17] Hirata DB, Albuquerque TL, Rueda N, Virgen-Ortiz JJ, Tacias-Pascacio VG, Fernandez-Lafuente R, J. Mol. Catal. B-Enzym., 133, 117 (2016)

[18] Barros M, Fleuri LF, Macedo GA, Braz J. Chem. Eng., 27(1), 15 (2010)

[19] Boukid F, Folloni S, Ranieri R, Vittadini E, Trends Food Sci Tech., 78, 120 (2018)

[20] Dlugy C, Wolfson A, Bioproc Biosyst Eng., 30(5), 327 (2007)

[21] Oh S, Park C, Enzyme Microb. Technol., 69, 19 (2015)

[22] Wong WC, Basri M, Razak CNA, Salleh AB, J. Am Oil Chem. Soc., 77(1), 85 (2000)

[23] Pierozan MK, da Costa RJ, Antunes OA, Oestreicher EG, Oliveira JV, Cansian RL, Treichel H, de Oliveira D, J. Agr. Food Chem., 57(20), 9716 (2009)

[24] Avelar MH, Cassimiro DM, Santos KC, Domingues RC, de Castro HF, Mendes AA, Ind. Crop. Prod., 44, 452 (2013)

[25] Soares CMF, De Castro HF, De Moraes FF, Zanin GM, Appl. Biochem. Biotechnol., 77-79, 745 (1999)

[26] Verdasco-Martin CM, Garcia-Verdugo E, Porcar R, Fernandez-Lafuente R, Otero C, Food Chem., 245, 39 (2018)

[27] Chakraborty R, Mukhopadhyay P, Kumar B, Energy Conv. Manag., 126, 32 (2016)

[28] Tudorache M, Protesescu L, Coman S, Parvulescu VI, Green Chem., 14(2), 478 (2012)

[29] Jung H, Lee Y, Kim D, Han SO, Kim SW, Lee J, Kim YH, Park C, Enzyme Microb. Technol., 51(3), 143 (2012)

[30] Devendran S, Yadav GD, Chirality, 26(6), 286 (2014)

[31] Taher H, Al-Zuhair S, Biofuel. Bioprod. Bior, 11(1), 168 (2017)

[32] Teng WK, Ngoh GC, Yusoff R, Aroua MK, Energy Conv. Manag., 88, 484 (2014)

[33] Paula AV, Urioste D, Santos JC, de Castro HF, J. Chem. Technol. Biotechnol., 82(3), 281 (2007)

[34] Jiang H, Zhang Y, Wang X, Ind. Crop. Prod., 30(2), 304 (2009)