Liquid-liquid interface bioreactor (L-L IBR) is a unique non-aqueous bioconversion system which comprises a hydrophobic organic solvent (upper phase), a fungal cells floating microspheres (MS) layer (middle phase), and a liquid medium (lower phase). In this study, a modified L-L IBR with actinomycetes and yeasts was developed by using binder micro-pieces (BM) and estimated its availability through some bioconversions. This modified interface cultivation system was named a tacky liquid liquid interface bioreactor (L-L IBRtac). After the detailed estimation of its characteristics, the system was applied to oxidation of citronellol to citronellal, 2-methylcyclohexanol to 2-methylcyclohexanone, and 2-octanol to 2-octanone with Rhodococcus hoagii NBRC 3730, oxidation of citronellal to citronellic acid with Candida viswanathii NBRC 10321, and transacetylation of citronellol by acetyl coenzyme A (acetyl-CoA) produced from glucose by Pichia kluyveri NBRC 1165. The accumulation of citronellal, 2-methylcyclohexanone, and 2-octanone reached 3.1 (16 days), 2.3 (12 days), and 32.9 g/l (12 days) in spite of strong biotoxicities of the substrates/products without collapse of a cells-MS-BM layer. On the other hand, 6.1 g/l of citronellic acid and 2.8 g/l of citronellyl acetate were produced from 5% citronellal and 10% citronellol for 12 days, respectively.