This study experimentally investigated the electrostatic characteristic of L-isoleucine powder, which is an amino acid powder. L-isoleucine is a crystal powder that has been used in industrial production sites. The volume resistivity, charge-to-mass ratio, and minimum ignition energy (MIE) or L-isoleucine powder were tested in this experiment. The results show that L-isoleucine powder had a volume resistivity as high as 1.2 x 1013 Omega m, classified as highly electrifying, with a high risk of electrostatic charge. The actual charge-to-mass ratio was also as high as 6.4 nC/g on average. Added to this, with a MIE of 4 mJ, it is a material very sensitive to electrostatic discharge. (C) 2019 Elsevier B.V. All rights reserved.