Soil washing, which is used to remove heavy metals from soil, is dependent on suitable washing agents. However, there is still a lack of economical, environmentally friendly washing agents with high removal efficiency. In this study, three washing agents, carboxyalkylthiosuccinic acid (CETSA), copolymer of maleic and acrylic acid (MA/AA) and ethylenediamine tetra acetic acid (EDTA), were used to remove heavy metals from contaminated soil. The influence of washing solution concentration, pH and washing time on heavy metals removal was also investigated. The cadmium (Cd), lead (Pb), and zinc (Zn) removal efficiencies increased as washing solution concentrations increased from 0 to 60 g L-1, while they declined as pH increased from 3 to 8. Despite fluctuations between 90 and 120 min, heavy metal removal efficiencies increased continuously from 10 to 90 min. The three agents also effectively reduced the potential risks of Cd, Pb, and Zn in contaminated soil, but only CETSA and MA/AA produced no significant changes in chemical properties. Fourier transform infrared spectroscopy revealed that the hydroxyl, carboxyl, carbonyl, methoxyl, and sulfur groups were related to the heavy metal ions from the soil colloids. Thus, CETSA and MA/AA were suitable washing agents for remediation of heavy metals contaminated soil.