Photoactive coatings of sol-gel ZnO suspensions were electrosprayed on glass substrates to produce self-cleaning antimicrobial functionalized surfaces. ZnO-functionalized materials exhibited a uniform external surface consisting of a pattern of microspheres with diameters in the 100-300 nm range. Electrospray allowed surface densities up to 0.30 mg cm(-2) that displayed considerable hydrophilicity. Water contact angle decreased with UV irradiation to values below 10 degrees. Two different UV doses were tested by adjusting the irradiation time to simulate Summer-Spring and Winter-Fall conditions. The functionalized coatings showed excellent photocatalytic properties towards the photodegradation of Methylene blue. The electrosprayed surfaces also displayed antibacterial activity against Staphylococcus aureus, with > 99.5% reduction in the number of culturable cells. The biocidal activity is attributed to the photogenerated reactive oxygen species on the surface of ZnO coatings and the bioavailable zinc ions produced from ZnO dissolution. The photoactive coatings kept surfaces free from bacterial colonization and biofilm formation.