Copper hexacyanoferrate-polypyrrole (CuHCF-PPy) nanocomposite as nicotine electrochemical sensor was deposited directly on reduced graphene oxide (rGO) by a direct self-assembly technique for the first time. FTIR and EDX confirmed the formation of CuHCF-PPy whereas combination of cubic CuHCF-PPy globules morphology was determined by FESEM. The electrochemical properties were studied via CV and its equivalent circuit fitted from EIS was described in details. The linear range of the proposed sensor was between 0.03 and 5 mM, which is the highest among the previous report, with a compatible sensitivity of 0.21 mA.cm(-2) mM(-1). Additionally, the sensor was applied for the detection of nicotine content in vape (e-cigarette) real sample.