Biomass of high-yielding strains of phototrophic microorganisms actively accumulating lipids is a promising non-traditional raw material for bioenergy including the production of biodiesel. In this study, we present results of searching for new strains of microalgae-producers of lipids from hot springs. Within the framework of research, the primary screening of water for the presence of lipid - accumulative microalgae was carried out with the help of qualitative reaction with lipid-specific dyes, as well as 5 axenic isolates of microalgae with stable growth were identified in the laboratory and their productivity and fatty acid composition were studied. The isolated strains were identified as Chlorella vulgaris sp-1, Ankistrodesmus sp-21, Scenedesmus obliquus sp-21, Chlorella pyrenoidosa sp-13 and Chlamydomonas sp-22. The obtained data showed that the isolated strains determined by biomass in the range 1.3 g/l to 1.81 g/l. As a result of the research, it was established that the highest content of lipids was observed in the strains Chlorella vulgaris sp-1 and Scenedesmus obliquus sp-21, which is 28.7 and 29.8% of the cell dry weight, respectively. The analysis of the fatty acid composition of the cells showed that the largest mass fraction of saturated and monounsaturated fatty acids was found in strain Scenedesmus obliquus sp-21 - 61.9%. In the result, Scenedesmus obliquus sp-21 strain isolated from thermal sources was selected as a promising candidate for biodiesel production. (C) 2019 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.