상어 연골 추출물이 함유된 구내염 치료용 하이드로젤의 개발

박은지; 국민석; 김 진; 이기영; Eun-Jee Park; Min-Suk Kook; Jin Kim; Ki-Young Lee

Polymer(Korea), Vol.43, No.3, 365-371, May, 2019

DOI10.7317/pk.2019.43.3.365 Export Citation

상어 연골 추출물이 함유된 구내염 치료용 하이드로젤의 개발

Development of Shark Cartilage Extracts Loaded Hydrogel for the Stomatitis Treatment

박은지, 국민석¹, 김 진^{2, †}, 이기영 ^{3, †}

전남대학교 신화학소재공학과
¹전남대학교 치의학 전문대학원 구강악안면외과학교실
²조선대학교 치과대학 구강악안면외과학교실 & 조선대학교 치의학연구원
³전남대학교 화학공학부

Eun-Jee Park, Min-Suk Kook¹, Jin Kim^{2, †}, and Ki-Young Lee^{3, †}

Department of Advanced Chemicals and Engineering, Chonnam National University, Gwangju 61186, Korea
¹Department of Oral and Maxillofacial Surgery, Chonnam National University School of Dentistry, Gwangju 61186, Korea
²Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University & Institute of Dental Science, Chosun University, Gwangju 61452, Korea
³School of Chemical Engineering, Chonnam National University, Gwangju 61186, Korea

E-mail:,

초록

구내염 치료를 위해 상어 연골 추출물(SCE)을 포함하는 poly(vinyl alcohol)(PVA) 하이드로젤을 동결-융해 방법으로 제조하였다. 하이드로젤의 특성은 FTIR(Fourier transform infrared spectroscopy), EDS(energy-dispersive Xray spectroscopy), 표면 pH, 접촉각, 생체점착강도를 통해 평가하였다. PVA/SCE 하이드로젤은 PVA 하이드로젤보다 구강점막에 대해 점막점착력이 있는 것을 확인하였다. 시리안 햄스터에 구강궤양을 유발한 후 PVA/SCE(shark cartilage extracts) 하이드로젤을 처치했을 때 대조군 그룹보다 높은 궤양 치유 효과를 확인하였다.

Poly(vinyl alcohol) (PVA) hydrogel containing shark cartilage extract was prepared by freezing-thawing method for the treatment of stomatitis. The properties of hydrogel were evaluated by FTIR (Fourier transform infrared spectroscopy), EDS (energy-dispersive X-ray spectroscopy), surface pH, contact angle, and bio-adhesive strength. PVA/ SCE hydrogel was found to have adhesion in the oral mucous membrane. PVA/SCE (shark cartilage extracts) hydrogel was attached to a hamster with oral ulcers. We have identified a higher ulcer healing effect with the treatment of the PVA/SCE hydrogel than the control group.

Keywords:poly(vinyl alcohol);hydrogel;shark cartilage;stomatitis;oral delivery system

[References]

Chavan M, Jain H, Diwan N, Khedkar S, Shete A, Durkar S, J. Oral. Pathol. Med., 41, 577 (2012)
Preeti L, Magesh K, Rajkumar K, Karthik R, J. Oral. Maxillofac. Pathol., 15, 252 (2011)
Zhou Y, Chen Q, Meng W, Jiang L, Wang Z, Liu J, Lin M, Zhou H, Chen X, Zhao M, Zeng X, Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod., 109, 561 (2010).
Altenburg A, El-Haj N, Micheli C, Puttkammer M, Abdel-Naser MB, Zouboulis CC, Dtsch. Arztebl. Int., 111, 665 (2014)
Femiano F, Buonaiuto C, Gombos F, Lanza A, Cirillo N, Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod., 109, 402 (2010).
Volkov I, Rudoy I, Freud T, Sardal G, Naimer S, Peleg R, Press Y, J. Am. Board. Fam. Med., 22, 9 (2009)
Liang MW, Neoh CY, Ann. Acad. Med.. Singap., 41, 463 (2012)
Nguyen S, Hiorth M, Ther. Deliv, 6, 595 (2015)
Andrews GP, Laverty TP, Jones DS, Eur. J. Pharm. Biopharm., 71, 505 (2009)
Nikalje AP, Tiwari S, Kamble S, Int. J. Res. Pharm. Sci., 2, 32 (2012)
Tangri P, Madhav N, JBI, 2229, 7499 (2011)
Peppas NA, Mongia NK, Eur. J. Pharm. Biopharm., 43, 51 (1997)
Mandal TK, Eur. J. Pharm. Biopharm., 50, 337 (2000)
Peppas NA, Scott JE, J. Control. Release, 18, 95 (1992)
Morimoto K, Fukanoki S, Morisaka K, Hyon SH, Ikada Y, Chem. Pharm. Bull., 37, 2491 (1989)
Morimoto K, Nagayasu A, Fukanoki S, Morisaka K, Hyon SH, Ikada Y, Pharm. Res., 6, 338 (1989)
Korsmeyer RW, Peppas NA, J. Membr. Sci., 9, 211 (1981)
Peppas NA, Mongia NK, Eur. J. Pharm. Biopharm., 43, 51 (1997)
Peppas NA, Berner RE, Biomater Res., 1, 158 (1980)
Korsmeyer RW, Peppas NA, J. Membr. Sci., 9, 211 (1981)
Gimenez V, Mantecon A, Cadiz V, J. Appl. Polym. Sci., 59(3), 425 (1996)
Gimenez V, Mantecon A, Cadiz V, J. Polym. Sci. A: Polym. Chem., 34(6), 925 (1996)
Bo J, J. Appl. Polym. Sci. Symp., 46, 783 (1992)
da Costa Sanches SC, de Vasconcelos F, da Costa CEF, et al., J. Therm. Anal. Calorim., 120, 991 (2015)
Ohtsuka Y, Nakae H, Abe H, Obinata T, Zoolog. Sci., 11, 407 (1994)
Mollica L, De Marchis F, Spitaleri A, Dallacosta C, Pennacchini D, Zamai M, Agresti A, Trisciuoglio L, Musco G, Bianchi ME, Chem. Biol., 14, 431 (2007)
Prasanna RI, Sankari KU, Int. J. Pharm. Investig., 2, 26 (2012)
Pavia DL, Lampman GM, Kriz GS, Vyvyan JA, Introduction to Spectroscopy, 4th Edition, California, Brookescole Pubishers, 2008.
Chen J, Cyr G, Composition producing adhesion through hydration in mainly Adhesion in biological system, New York, Academic Press, 1970.
Duchene D, Touchard F, Peppas N, Drug Dev. Ind. Pharm., 14, 283 (1988)
Barrer R, Barrie J, Wong PL, Polymers, 9, 609 (1968)
Shaikh TRRSR, Garland MJ, Woolfson AD, Donnelly RF, J. Pharm. Bioallied. Sci., 3, 89 (2011)
Kalyanaraman B, Redox Biol., 1, 244 (2013)

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[1] Chavan M, Jain H, Diwan N, Khedkar S, Shete A, Durkar S, J. Oral. Pathol. Med., 41, 577 (2012)

[2] Preeti L, Magesh K, Rajkumar K, Karthik R, J. Oral. Maxillofac. Pathol., 15, 252 (2011)

[3] Zhou Y, Chen Q, Meng W, Jiang L, Wang Z, Liu J, Lin M, Zhou H, Chen X, Zhao M, Zeng X, Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod., 109, 561 (2010).

[4] Altenburg A, El-Haj N, Micheli C, Puttkammer M, Abdel-Naser MB, Zouboulis CC, Dtsch. Arztebl. Int., 111, 665 (2014)

[5] Femiano F, Buonaiuto C, Gombos F, Lanza A, Cirillo N, Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod., 109, 402 (2010).

[6] Volkov I, Rudoy I, Freud T, Sardal G, Naimer S, Peleg R, Press Y, J. Am. Board. Fam. Med., 22, 9 (2009)

[7] Liang MW, Neoh CY, Ann. Acad. Med.. Singap., 41, 463 (2012)

[8] Nguyen S, Hiorth M, Ther. Deliv, 6, 595 (2015)

[9] Andrews GP, Laverty TP, Jones DS, Eur. J. Pharm. Biopharm., 71, 505 (2009)

[10] Nikalje AP, Tiwari S, Kamble S, Int. J. Res. Pharm. Sci., 2, 32 (2012)

[11] Tangri P, Madhav N, JBI, 2229, 7499 (2011)

[12] Peppas NA, Mongia NK, Eur. J. Pharm. Biopharm., 43, 51 (1997)

[13] Mandal TK, Eur. J. Pharm. Biopharm., 50, 337 (2000)

[14] Peppas NA, Scott JE, J. Control. Release, 18, 95 (1992)

[15] Morimoto K, Fukanoki S, Morisaka K, Hyon SH, Ikada Y, Chem. Pharm. Bull., 37, 2491 (1989)

[16] Morimoto K, Nagayasu A, Fukanoki S, Morisaka K, Hyon SH, Ikada Y, Pharm. Res., 6, 338 (1989)

[17] Korsmeyer RW, Peppas NA, J. Membr. Sci., 9, 211 (1981)

[18] Peppas NA, Mongia NK, Eur. J. Pharm. Biopharm., 43, 51 (1997)

[19] Peppas NA, Berner RE, Biomater Res., 1, 158 (1980)

[20] Korsmeyer RW, Peppas NA, J. Membr. Sci., 9, 211 (1981)

[21] Gimenez V, Mantecon A, Cadiz V, J. Appl. Polym. Sci., 59(3), 425 (1996)

[22] Gimenez V, Mantecon A, Cadiz V, J. Polym. Sci. A: Polym. Chem., 34(6), 925 (1996)

[23] Bo J, J. Appl. Polym. Sci. Symp., 46, 783 (1992)

[24] da Costa Sanches SC, de Vasconcelos F, da Costa CEF, et al., J. Therm. Anal. Calorim., 120, 991 (2015)

[25] Ohtsuka Y, Nakae H, Abe H, Obinata T, Zoolog. Sci., 11, 407 (1994)

[26] Mollica L, De Marchis F, Spitaleri A, Dallacosta C, Pennacchini D, Zamai M, Agresti A, Trisciuoglio L, Musco G, Bianchi ME, Chem. Biol., 14, 431 (2007)

[27] Prasanna RI, Sankari KU, Int. J. Pharm. Investig., 2, 26 (2012)

[28] Pavia DL, Lampman GM, Kriz GS, Vyvyan JA, Introduction to Spectroscopy, 4th Edition, California, Brookescole Pubishers, 2008.

[29] Chen J, Cyr G, Composition producing adhesion through hydration in mainly Adhesion in biological system, New York, Academic Press, 1970.

[30] Duchene D, Touchard F, Peppas N, Drug Dev. Ind. Pharm., 14, 283 (1988)

[31] Barrer R, Barrie J, Wong PL, Polymers, 9, 609 (1968)

[32] Shaikh TRRSR, Garland MJ, Woolfson AD, Donnelly RF, J. Pharm. Bioallied. Sci., 3, 89 (2011)

[33] Kalyanaraman B, Redox Biol., 1, 244 (2013)