This study investigates the use of anodization in NH4F/NaCl electrolyte in the formation of self-assembled nanotube arrays on a Ti-7.5Mo surface. As anodic oxidation potential increased from 5 V to 10 V, the pore size and tube length of the nanotubes gradually increased approximately from 23-27 to 31-44 nm, and 550 to 700 nm. The XRD results indicated that the oxide layers on the surface of the anodized samples were all amorphous structures. After annealing at 450 degrees C for 3 h, peaks of anatase phase were observed. The results of the XPS analysis showed that the major components on the surfaces of the annealed nanotube arrays were TiO2, MoO2, and MoO3. In vitro testing of the annealed nanotube arrays demonstrated that rapid deposition of apatite spheroids on these nanostructures occurred after only 12 h immersion in simulated body fluids (SBF), a result that was particularly evident in the samples anodized at 10 V. Cell culture experiments showed that the anodized Ti-7.5Mo alloy is biocompatible and can support cell attachment.