A polymeric film sensor containing poly(azomethine-urethane) was easily fabricated using the dip-coating technique and ion sensing property of this film toward different metal cations investigated. Fluorescence measurements showed that fluorescent film sensor has been exhibited dual emission wavelengths at 540 and 582 nm and metal cation sensing behavior of this film studied using these two wavelengths. Also, fluorescent film sensor was showed selectivity and sensitivity toward iron (III) ions in deionized water with excellent photostability. The detection limit of the polymeric sensor was found as 86.15 and 28.90 mu M at these wavelengths. The results indicated that chemosensor can be successfully applied for the detection of iron (III) ions in deionized water. Contact angle measurements of the film probe were also evaluated to investigate the surface hydrophilicity or hydrophobicity of the film.