An atmospheric pressure glow discharge generated between the solid cathode and liquid anode was applied to produce silver nanoparticles and deposition of them onto a polymeric substrate. The formation of nanoparticles in liquid solution was confirmed by UV-Vis absorbance spectroscopy and dynamic light scattering method. The activation of polymer surfaces was detected using FTIR spectroscopy. The deposition of nanostructures onto polymeric substrates was confirmed by atomic force microscopy and scanning electron microscopy. It has been shown that forming a colloid system is a poor stability that results in deposition/sedimentation of nanoparticles. The distribution of nanostructures on the polymer surface is non-uniform. The possible mechanism of fixation of silver nanoparticles on the surface of polymer films suggested.