A novel carboxyl-group functionalized silicate nanopore films (SNFs) was successfully fabricated on Pt electrode by immobilization of the composite material containing polyacrylic acid (PAA) and tetraethyl orthosilicate (TEOS). The composite films have relatively uniform pores with an average of 20 nm in diameter and are endowed with a great amount of carboxyl groups, which provided large amount of negative charge on film surface. Scanning electron microscope (SEM), Transmission electron microscope (TEM) images, EDS spectrum and FTIR spectrum have been used to characterize the prepared SNFs. Three redox species with different charge, including hexacyanoferrate (Fe(CN)(6)(3-)), hexaamino-ruthenium (Ru(NH3)(6)(3+)) and ferrocene methanol (FcMeOH) in different pH, were employed to investigate the permselectivity of the SNFs modified electrode. The results showed that the SNFs modified electrode favored the mass transport of oppositely charged species (Ru(NH3)(6)(3+)), repelled similarly charged species (Fe(CN)(6)(3-)), and had little effect on the transport of neutral species (FcMeOH). Based on this good permselective property, the prepared SNFs modified electrode was employed to detect dopamine (DA) in the presence of ascorbic acid (AA) at pH 7.6. The prepared SNFs electrode exhibited a good sensing performance for detection of DA with the linear range of 10-1900 mu M and the detection limit of 1.7 mu M (S/N = 3). (C) 2019 The Electrochemical Society.