The herbicide Triclopyr (TCP) is widely applied to minimize the growth of woody plants. Monitoring of TCP is of major environmental concern due to its adverse impact on aquatic organisms, soils and animals. Electrochemical behavior of TCP was investigated at C-70 decorated PANI modified glassy carbon sensor (PANI/C-70/GC). The experimental parameters, such as concentration, pH, amplitude, frequency, deposition potential were optimized. The modified sensor exhibited an excellent catalytic response towards the reduction of TCP with a well-defined reduction peak at 1.72 V. The developed sensor was characterized by scanning electron microscopy (SEM), fourier transform infrared spectroscopy (VIM), X-Ray diffraction (XRD) and electrochemical impedance spectroscopy (EIS). The electrochemical measurements were carried out using square wave (SWV) and cyclic voltammetry (CV). The modified sensor exhibited linear calibration curve for TCP over a concentration range of 10 ngmL(-1)-100 ngmL(-1) with detection limit of 1.9 ngmL(-1). The developed sensor could detect TCP efficiently without any interference from the common metabolites. The voltammetric procedure was applied successfully to real sample analysis with high sensitivity and good selectivity.