The aim of the study was to compare the effects of different remineralization methods that are well established in clinical and daily use on S. mutans biofilm adhesion. In this study 72 human third molars were used. From each tooth two pieces of 4 mm x 7 mm enamel blocks were acquired. The samples were divided into 6 groups in which include 10 samples per time period (24h and 48 h) and for each remineralization method; control, flouride, ozone, CPP-ACP, arginine, novamin. After remineralization procedures, enamel surfaces were covered with saliva. 10(5) CFU/mL of active S. mutans culture were inoculated onto the samples. S. mutans colonies were counted with Plate Count Agar (PCA) decimal dilution method. Micromorphologic effects of different remineralization methods were observed by Scanning Electron Microscopy (SEM). The most S. mutans biofilm formation for both time periods was observed in the control group whereas the less biofilm adhesion was showed in the arginine group. There were no statistically significant differences among remineralization agents (p > 0.05). In the control group there was statistical difference between 24 h and 48 h (p < 0.005) but in the other study groups there were no significant difference between the time periods (p > 0.05). Remineralization agents did not significant differ on S. mutans biofilm adhesion.