This study aimed to use sweet corn hybrid hi-brix53 stalk juice for bioethanol production, to give a solution to the growing problem of food vs. fuel and to utilize waste for cheaper production. Hi-brix 53 stalk juice contained 112.07 +/- 2.99 g L-1 of total sugars and 21.83 +/- 1.09 g L-1 of reducing sugars. Through fermentation (24-120 h) using yeast (Saccharomyces cerevisiae), it produced 6.01% (v/v) bioethanol. The final ethanol produce (g L-1) yield efficiency and volumetric ethanol productivity were at the highest at 24 h with 47.87 L-1, 87.62% and 1.97 +/- 0.06 (g L-1 h(-1)). These results suggest that hi-brix 53 stalk juice is an ideal substrate for bioethanol production.