In this work, florfenicol removal via two kinds Chlorella sp. (UTEX1602 and L38) was investigated. The experimental results indicated that FF could be removed by biodegradation associated with microalgae growth. Compared to Chlorella sp. UTEX1602, L38 had a good self-adjustment capacity at the condition of high initial FF concentration. The biodegradation of FF followed the first order kinetic model with half-lives ranged from 3.53 to 7.63 days at different initial concentration. The removal efficiency of FF could achieve 97% when the FF concentration was set at 46 mg.L-1. While the FF concentration in the medium increased to 159 mg.L-1, more than 74% FF could still be purified via Chlorella sp. L38. Therefore, Chlorella sp. L38 could be promising alternative algae to be used for FF removal from different water sources.