The main aim of this study was to optimize pretreatment strategies of Miscanthus x giganteus for biosuccinic acid production. A successful pretreatment with organosolv method ( 80% w/w of glycerol, 1.25% of H2SO4), prevented sugars conversion to furfurals and organic acids, and thereby resulted in high sugar recovery ( glucan > 98%, xylan > 91%) and biomass delignification ( 60%). Pretreated biomass was subjected to hydrolysis with various cellulolytic enzyme cocktails ( Viscozyme (R) L, Carezyme 1000L (R), beta-Glucanase, Cellic (R) CTec2, Cellic (R) HTec2). The most effective enzymes mixture composed of Cellic (R) CTec2 ( 10% w/w), beta-Glucanase ( 5% w/w) and Cellic (R) HTec2 ( 1% w/w) resulted in high glucose ( 93.1%) and xylose ( 69.2%) yields after glycerol-based pretreatment. Succinic acid yield of 75-82% was obtained after hydrolysates fermentation, using Actinobacillus succinogenes 130Z. Finally a successful downstream concept for succinic acid purification was proposed. The succinic acid recovery with high purity (> 98%) was developed.