Urothelial Carcinoma Antigen 1 (UCA1) is a cell and tissue specific long non-coding RNA (lncRNA) associated with the tumorigenesis and invasion of bladder cancer. However, the mechanism driving the over-transcription of UCA1 in bladder cancer cells remains unclear. It has been reported that C/EBP beta has a significant role of regulation in tumorigenesis. Here we report that the expression of UCA1 was dramatically inhibited in 5637 cells with C/EBP beta down-regulation. Additionally, the function tests indicated that C/EBP beta could promote 5637 cells growth and colony formation by inducing the expression level of UCAl. These data suggest that C/EBP beta was involved in transcriptional regulation of UCA1 and contributed substantially to its high expression and proliferation promoting in bladder cancer cells. (C) 2018 Elsevier Inc. All rights reserved.