AtMYB44 has been described in diverse hormonal signaling processes including abscisic acid (ABA)- mediated tolerance to abiotic stress; however, its function as a transcription factor is controversial. AtMYB44 contains the amino acid sequence LAUX1 acts upstream of PIN2 in regulating root gravitropism S (L) under barS (L) under barS (L) under bar, a putative ETHYLENE-RESPONSIVE ELEMENT BINDING FACTOR-ASSOCIATED AMPHIPHILIC REPRESSION (EAR) motif. In yeast two-hybrid assay, physical interaction between AtMYB44 and a TOPLESS-RELATED (TPR) corepressor was observed, but abolished by mutation of the EAR motif. We performed bimolecular fluorescence complementation assay to confirm their interaction in planta. Chromatin immunoprecipitation assay revealed binding of AtMYB44 to the promoter regions of clade A protein phosphatase 2C (PP2C) genes (e.g., ABI1, ABI2, and HAI1), implying putative targets. Levels of histone H3 acetylation around the promoter regions were markedly lower in AtMYB44-overexpressing (35S:AtMYB44) plants than in wild-type plants. These results suggest that AtMYB44 forms a complex with TPR corepressors and recruits histone deacetylase(s) to suppress PP2C gene transcription in a signal-independent manner. (C) 2018 Elsevier Inc. All rights reserved.