With co-treatment of potassium oxonate (PO) and xanthine sodium salt (XSS), a zebrafish larva model of acute hyperuricemia has been constructed for the first time. The results show PO 200 mu M + XSS 10 mu M, PO 300 mu M + XSS 15 mu M, and PO 400 mu M + XSS 20 mu M can significantly increase the level of uric acid in the zebrafish larvae (P < 0.05), the concentrations as described above can be used to construct the zebrafish larvae model of acute hyperuricemia. At the same time, treatment of allopurinol (APL, one of the hyperuricemia drugs) at 2000 mu M (P < 0.001) and treatment of anserine (ASE) at 200 mu M (P < 0.05) could significantly decrease the level of uric acid in the model group which received PO 200 mu M + XSS 10 mu M, which demonstrate that such model could offer a new robust approach for high-throughput screening of food and drugs with uric acid-lowering activity. (C) 2018 Published by Elsevier Inc.