Disruption of cell cell junction and adhesion to vessels are crucial steps in tumor metastasis. Tumor-associated glycoprotein 72 (TAG-72) is a crucial membrane mucin in gastroesophageal mucosa for microenvironment contact with cells. Thus, the TAG-72 value may be an indicator of the malignant involvement of lymph nodes in esophagogastric junction adenocarcinoma (EGAC) patients. Of the 183 patients suspected as gastroesophageal neoplasms, 129 were subsequently diagnosed as EGAC, and 54 were subsequently diagnosed as benign gastroesophageal diseases by imageological or/and histological examination. After we obtained preoperative serum TAG-72 values, the relationship between serum TAG-72 and lymphatic metastasis status, extent of invaded lymph nodes and clinical stage was tested using Spearman correlation analysis and chi(2) tests. Compared with those in patients who suffered either benign gastroesophageal diseases or preinvasive carcinoma, the median serum TAG-72 values were statistically higher in EGAC patients with positive lymph nodes (Kruskal-Wallis test; P < 0.001). Serum TAG-72 values were significantly correlated with Lymph Node Ratio (LNR) (Spearman correlation; P < 0.001). Using corresponding ROC (95% CI = 0.621-0.783, P < 0.0001), serum TAG-72 values with an optimal cut-off (2.2 kU/mL) showed a sensitivity of 0.632 and a specificity of 0.690 for predicting malignant lymph node involvement in EGAC. These results suggest that the serum TAG-72 value is a clinically helpful predictor of lymph nodes invasion in resectable EGAC. (C) 2018 The Authors. Published by Elsevier Inc.