In recent years, Ge nanomaterials have aroused a great deal of attention because of their unique physical and chemical properties. However, the current synthesis methods bear some disadvantages, such as high reaction temperature, dangerous reagents, and inert atmospheres. In this paper, we developed a facile one-step route for preparing fluorescent and water-dispersed germanium nanoparticles (Ge NPs) by utilizing organogermanes as the precursor, operated at mild reactive conditions. The as-synthesized Ge NPs have an average diameter of 2.6 +/- 0.5 nm and intense blue-green fluorescence (FL). Furthermore, the as-synthesized Ge NPs show remarkable water dispersibility, favorable biocompatibility, outstanding photostability, excellent storage stability, and low cytotoxicity. More importantly, these Ge NPs can act as a satisfactory FL probe and successfully be applied to cellular imaging of HeLa. The present study offers a simple and moderate strategy for the preparation of Ge NPs and expedites Ge NPs for bioimaging applications.