A simple and scalable method was proposed for the preparation of mesoporous membranes by assembling two-dimensional (2D) MXene nanosheets and TiO2 nanoparticles (NPs) on a macroporous support. MXene nanosheets were introduced into TiO2 hydrosol, and then the sol was transformed into gel for fabrication of mesoporous TiO2-MXene membranes. Characterization results confirmed that an assembled structure of TiO2-MXene nanosheets was formed, benefiting from the nanosheets as 'floor tiles', thereby successfully inhibited the sol infiltration to the porous support. As a result, the hydrosols could be directly coated on macroporous substrates (with only one single cycle of aging and calcination) such as a disc support with an average pore size of similar to 100 nm and a four-channel hollow fiber with an average pore size of similar to 00 nm. Filtration experiments revealed the resulted membranes, in particular of the hollow fiber TiO2-MXene membranes, had a narrow pore size distribution, desirable rejection characteristics of dextran and high water permeate flux. The 2D nanosheet assisted TiO2 NPs assembly proposed in this work promises a simple, feasible and scalable approach for fabricating high-performance mesoporous TiO2 membranes.