A novel silver nanowire/nickel-cobalt layered double hydroxide (AgNW/NiCo LDH) nanocomposite was successfully synthesized, after which a glucose sensor was fabricated. AgNW was synthesized by ethylene glycol as reductant in presence of Fe3+, and NiCo LDH grafted vertically along the 1D AgNW by slow hydrolysis of urea. The morphology and composition of AgNW/NiCo LDH were characterized by Scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and Fourier transform infrared spectroscopy. The electrochemical investigation showed that the AgNW/NiCo LDH/GCE exhibited excellent electrocatalytic ability to glucose oxidation, with a linear range from 0.002 mM to 6 mM at a low applied potential of 0.4 V (vs. SCE) and a low detection limit of 0.66 mu M at the signal-to-noise ratio of 3. It is expected that the AgNW/NiCo LDH nanocomposites appear to be a promising catalyst for non-enzymatic glucose sensor.