The aim of this study is to determine the availability of pomegranate seed oil biodiesel (POB) as an alternative fuel in diesel engines and evaluate engine performance and emission characteristics of pure hydrogen enriched POB using diesel engine. For this purpose, the intake manifold of the test engine was modified and hydrogen enriched intake air was supplied throughout the experiments. Physical properties of POB and its blend with diesel fuel were also determined. The results showed that measured physical properties of POB are comparable with diesel fuel. According to engine performance experiments, although POB utilization has slight undesirable effects on some engine performance parameters such as brake power output and specific fuel consumption, it can be used as alternative fuel in diesel engines, by this way CO emission can be improved. Finally, hydrogen enrichment experiments indicated that pure hydrogen addition causes a slight improvement in both engine performance and exhaust emissions. (C) 2017 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.