Microfluidic microbial fuel cells (mu MPC) combine the microfluidic technology and biocatalysts anchored at the electrode, representing an emerging bio-power generator. However, a major challenge towards further development is their low bio-catalysts loading on the electrodes. Previous researches have revealed the bio-electrode structure and nutrient-feeding type play a significant role in the power generation of jtIvIFC. In this study, we fabricate a membrane-free LasAFC with a piece of graphene-decorated nickel mesh as bio-anode, which is fed in a flow-through manner. The scanning electron microscopy and pore-distribution variation analysis show the mixed bacteria grow densely on the surface of the electrode. Benefited from the enriched bacterial colonization and sufficient nutrient supply, the mu MFG produces a remarkably high areal power density of 1.11 W m(-2) at a current density of 2.15 A m(-2). These values are substantially higher than those of conventional two-dimensional electrodes and flow-over architectures measured under the same condition. (C) 2018 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.