This research focused on the removal of dibenzothiophene (DBT) from model diesel oil using CuO-loaded gamma-Al2O3-reduced graphene oxide (CuO/gamma-Al2O3-RGO) as an adsorbent, which possesses the highest adsorption capacity among reported regenerable graphene-related materials. The boehmite-RGO supports were synthesized using a one-pot hydrothermal synthesis method. As the RGO sheet surfaces are completely covered and isolated by boehmite nanorods formed via hydroxyl condensation of [Al(OH)(x)]((3-x)+) or Al(OH)(3) and the -OH of graphene oxide (GO), the specific surface area of boehmite-RGO increased to 378 m(2)/g, which is 20.8% higher than that of RGO (313 m(2)/g). CuO/gamma-Al2O3-RGO adsorbents with different CuO contents were prepared, and their DBT adsorption desulfurization properties were evaluated. With a 2% mass ratio of CuO to boehmite-RGO, CuO/gamma-Al2O3-RGO displays the highest DBT adsorption capacity (6.0 mg S/g) under ambient conditions, which improved by 53.8% in comparison with that of pure RGO (3.9 mg S/g). Moreover, the regeneration of the CuO/gamma-Al2O3-RGO adsorbent is much more practical than that of the pure RGO adsorbent.