This work investigates the suspension duration of the nanosized multiwalled carbon nanotubes (MWCNT) and aluminum oxide (Al2O3) in B20, B50 and B70 blends of Jatropha Methyl ester. The MWCNT and aluminum oxide (Al2O3) are added to the fuel blends in the proportions of 50 and 100pmm separately by ultra sonication. The prepared fuel samples are characterized, and turbidity analysis was done to find the stability rate of nano-additives. The outcomes reveal the maximum stability rate for MWCNT and Al2O3 as 83.3% and 87.03%, respectively, with 50ppm in B20 over a period of eighteen days. A considerable drop in suspension was observed with the 100ppm MWCNT and Al2O3 biodiesel blends.