In this study, a novel interlaced microchannel with a "cold water-hot water-cold water" counterflow arrangement was designed. The influences of microchannel configurations on the thermal and hydraulic performance were studied by comparing the proposed microchannel configuration with parallel and traditional spiral configurations. The results showed that the effective heat transfer area of the interlaced microchannel was 6.4 and 8.4 times that of the parallel and spiral configurations, respectively. For interlaced microchannels, the maximum temperature difference between the cross sections was 0.07 degrees C, and the temperature rise along the flow direction was only 6 degrees C. When the Reynolds number was 492, the Nusselt number of the interlaced microchannels was 2 and 10 times that of the parallel and spiral microchannels, respectively. The heat transfer performance of interlaced microchannels was improved by 83.46% compared with that in the literature. The influence of microchannel configurations on the pressure drop and the entrance length were negligible. The interlaced microchannel exhibited its lowest thermal resistance of 0.015 degrees C/W and lowest entropy production of 22.6 W/degrees C at a Reynolds number of 492. The heat transfer enhancement coefficient of the interlaced microchannel and parallel microchannel were 5 and 2.8 times that of the traditional spiral microchannel, respectively. The maximum heat load of loop heat pipe was enhanced by 4 times with the integration of interlaced microchannel as the condenser.